Hyaluronic acid-loaded p(HEMA) nanoparticles reduce the viability of SH-SY5Y neuroblastoma cells in a time-dependent manner
İlayda Manav1
, Selen Güneş2, Emre Vural2, Şeyma Eroğlu2, Elif Giçi2, Ayşegül Yıldız1
1Department of Molecular Biology and Genetics, Muğla Sıtkı Koçman University, Muğla, Türkiye
2Department of Graduate School of Natural and Applied Sciences, Muğla Sıtkı Koçman University, Muğla, Türkiye
Keywords: Controlled release, cytotoxicity, hyaluronic acid, nanoparticle, p(HEMA), SH-SY5Y.
Abstract
Objectives: This study aims to evaluate the effects of poly(2-hydroxyethyl methacrylate) [p(HEMA)]-based nanoparticles loaded with hyaluronic acid (HA) on the SH-SY5Y human neuroblastoma cell line.
Materials and methods: Three experimental groups were established: free HA, unloaded p(HEMA), and HA-loaded p(HEMA) nanoparticles. Treatments were applied for 12 and 24 h. Cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium (MTS) assay, which measures metabolic activity, and the resulting absorbance values were comparatively analyzed.
Results: According to the results, HA-loaded p(HEMA) nanoparticles did not exhibit a negative impact on cell metabolism at 12 h; however, a reduction in viability was observed at 24 h. These findings imply that prolonged release may contribute to cytotoxic effects. In contrast, free HA was found to exert a proliferative effect on the cells. For the unloaded p(HEMA) group, an initial decrease in cell viability was observed, which appeared to diminish over time.
Conclusion: These findings indicate that HA-containing carrier systems may elicit time- and content-dependent cellular responses and hold potential for neurological targeting.
Cite this article as: Manav İ, Güneş S, Vural E, Eroğlu Ş, Giçi E, Yıldız A. Hyaluronic acid-loaded p(HEMA) nanoparticles reduce the viability of SH-SY5Y neuroblastoma cells in a time-dependent manner. D J Med Sci 2025;11(2):67-73. doi: 10.5606/fng.btd.2025.181.
Conceptualization: A.Y., I.M.; Methodology: I.M., S.G., E.V., A.Y.; Validation: S.G., E.V., Ş.E.; Formal analysis: S.G., E.V., E.G.; Investigation: S.G., I.M.; Resources: E.V.; Data curation, visualization: Ş.E.; Writing-original draft: I.M., Ş.E., E.G., S.G.; Writing-review & editing: A.Y., Ş.E., S.G.; Supervision: A.Y.; Project administration: A.Y., S.G.; Funding acquisition: A.Y.
The authors declared no conflicts of interest with respect to the authorship and/or publication of this article.
This study was supported by the Scientific and Technological Research Council of Türkiye (TÜBİTAK) under the 2209-A Programme for Supporting Undergraduate Student Research Projects (Project No. 1919B012325269).
Data Sharing Statement:
The data that support the findings of this study are available from the corresponding author upon reasonable request.
We gratefully acknowledge the support of the Scientific and Technological Research Council of Türkiye (TÜBİTAK) under the 2209-A Programme for Supporting Undergraduate Student Research Projects (Project No. 1919B012325269). The views and conclusions expressed in this publication are those of the author(s) and do not necessarily reflect TÜBİTAK’s official position.