Apoptotic effect of epigallocatechin-gallate on C6 glioma cells

Hasan Arslanyüreği; Melike Ersöz; Tuncay Altuğ1*

doi:10.5606/fng.btd.2025.204

Hasan Arslanyüreği¹, Melike Ersöz², Tuncay Altuğ^1*

¹Department of Medical Biology and Genetics, Demiroğlu Science University, Institute of Health Sciences, İstanbul, Türkiye
²Department of Molecular Biology and Genetics, Demiroğlu Science University, Faculty of Arts and Sciences, İstanbul, Türkiye

Keywords: Apoptosis, C6 glioma, epigallocatechin-gallate, green tea, TUNEL.

Abstract

Objectives: In the present study, epigallocatechin-gallate (EGCG) was investigated for its ability to induce programmed cell death in rat-derived C6 glioma cells.

Materials and methods: The C6 glioma cells were cultured. Then the cells were incubated with 0 (Control), 50, and 100 μg/mL EGCG for 24, 48, and 72 h. After the treatment of EGCG, the apoptotic cell death was assayed using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method. The data obtained were analyzed using One-Way ANOVA.

Results: The study determined that EGCG, at concentrations of 50 and 100 μg/mL, induced apoptosis in C6 glioma cells after 24, 48, and 72 h. The most significant increase in apoptosis was observed in cells treated with 100 μg/mL EGCG for 72 h (p<0.001).

Conclusion: The present study demonstrates the apoptotic effect of EGCG on tumor cells, as assessed by TUNEL, supporting its potential as an alternative bioactive compound in cancer biology and therapeutic research. Given the molecular mechanisms of EGCG, combined treatment strategies for cancer therapies may be developed.

* Prof. Dr. Tuncay Altuğ made substantial contributions to the conception and design of the study and to the interpretation of the data. Sadly, he passed away prior to the submission of this manuscript.

Cite this article as: Arslanyüreği H, Ersöz M, Altuğ T. Apoptotic effect of epigallocatechingallate on C6 glioma cells. D J Med Sci 2025;11(3):144-149. doi: 10.5606/fng. btd.2025.204.